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quantibody mouse immunoglobulin isotype array  (RayBiotech inc)
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RayBiotech inc quantibody mouse immunoglobulin isotype array
Isotypes of early infection-induced antibodies and their kinetics in Balb/c GF mice sera and Balb/c SPF mice sera obtained 12, 24, and 48 h after F. tularensis FSC200 intraperitoneal infection, dose of 10 2 CFU in total volume of 0.2 mL of saline. The immunoglobulin subclasses of serum samples were determined using a <t>Quantibody</t> mouse immunoglobulin isotype array while following the manufacturer’s instructions. The antibody isotypes compositions of Balb/c SPF and GF murine sera obtained from naïve mice without any bacterial colonization were very low and are not shown in these graphs. The basal individual antibody isotypes levels for GF mice were significantly lower in comparison with the corresponding isotypes levels for SPF mice (data not shown). The Quantibody mouse immunoglobulin isotype arrays were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times. Reference isotypes are presented. Values are expressed as mean ± standard deviation (SD) and analyzed for significance using Student’s two-tailed t -test. Differences were considered statistically significant at **p < 0.01 and *p < 0.05 and are denoted by asterisks.
Quantibody Mouse Immunoglobulin Isotype Array, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Isotypes of early infection-induced antibodies and their kinetics in Balb/c GF mice sera and Balb/c SPF mice sera obtained 12, 24, and 48 h after F. tularensis FSC200 intraperitoneal infection, dose of 10 2 CFU in total volume of 0.2 mL of saline. The immunoglobulin subclasses of serum samples were determined using a Quantibody mouse immunoglobulin isotype array while following the manufacturer’s instructions. The antibody isotypes compositions of Balb/c SPF and GF murine sera obtained from naïve mice without any bacterial colonization were very low and are not shown in these graphs. The basal individual antibody isotypes levels for GF mice were significantly lower in comparison with the corresponding isotypes levels for SPF mice (data not shown). The Quantibody mouse immunoglobulin isotype arrays were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times. Reference isotypes are presented. Values are expressed as mean ± standard deviation (SD) and analyzed for significance using Student’s two-tailed t -test. Differences were considered statistically significant at **p < 0.01 and *p < 0.05 and are denoted by asterisks.

Journal: Scientific Reports

Article Title: Early infection-induced natural antibody response

doi: 10.1038/s41598-021-81083-0

Figure Lengend Snippet: Isotypes of early infection-induced antibodies and their kinetics in Balb/c GF mice sera and Balb/c SPF mice sera obtained 12, 24, and 48 h after F. tularensis FSC200 intraperitoneal infection, dose of 10 2 CFU in total volume of 0.2 mL of saline. The immunoglobulin subclasses of serum samples were determined using a Quantibody mouse immunoglobulin isotype array while following the manufacturer’s instructions. The antibody isotypes compositions of Balb/c SPF and GF murine sera obtained from naïve mice without any bacterial colonization were very low and are not shown in these graphs. The basal individual antibody isotypes levels for GF mice were significantly lower in comparison with the corresponding isotypes levels for SPF mice (data not shown). The Quantibody mouse immunoglobulin isotype arrays were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times. Reference isotypes are presented. Values are expressed as mean ± standard deviation (SD) and analyzed for significance using Student’s two-tailed t -test. Differences were considered statistically significant at **p < 0.01 and *p < 0.05 and are denoted by asterisks.

Article Snippet: The immunoglobulin subclasses of serum samples were determined using a Quantibody mouse immunoglobulin isotype array (RayBiotech Life, Peachtree Corners, GA, USA) while following the manufacturer’s instructions.

Techniques: Infection, Standard Deviation, Two Tailed Test